Current Issue : October-December Volume : 2015 Issue Number : 4 Articles : 5 Articles
A simple, rapid, accurate, precise and reproducible RP-HPLC method was developed and validated for the estimation of guaifenesin in tablet dosage form. The method was carried out using Enable C18 G 250 x 4.6 mm, 5 µ column in a binary mode with mobile phase water adjusted to pH 3 with OPA and acetonitrile in the ration of 73:27% v/v. Flow rate was set at 1.3 ml/min and detection was carried out at 240 nm using a PDA detector. The retention time was found to be 5.281min and the method produced linear response in the concentration range of 2-20 µg/ml (r2-0.999). The recovery studies were also carried out and % RSD from reproducibility was found to be below 2%. LOD and LOQ for this method were found to be 0.104 µg/ml and 0.318 µg/ml respectively. The developed method was simple, sensitive and specific. Hence the method can be used for the estimation of guaifenesin in tablet dosage form....
In the present study an economical, simple, precise, rapid, sensitive and accurate and reproducible Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method has been developed and validated for Lurasidone Hydrochloride. The chromatographic separation was carried out by using reverse phase stainless steel column of (4.6 mm × 25 cm) Purospher® STAR Hibar® C18 column with 5 µm (particles packing) held at 25°C. The mobile phase consisted of acetonitrile: Phosphate buffer (0.01M) (70:30 v/v), with a flow rate of 1.0 ml/ min and UV detection at 230 nm and injection volume 20 µL was maintained. The retention time was found to be 4.231 min. The different analytical performance parameters such as linearity, accuracy, precision and sensitivity (limit of detection and limit of quantitation) were determined according to International Conference on Harmonization ICH guidelines. The linearity was observed in the range of 10-100 µg/mL with correlation coefficient, r = 0.9963. The proposed method is rapid, cost-effective and can be used as a quality-control tool for routine quantitative analysis of Lurasidone HCl....
The present investigation was carried out for the estimation of sterol content in marketed edible oil and ghee samples. Eight samples of edible oils and ghee samples were subjected for sterol estimation. The samples include Ragava Palm Swathi (refined palm oil), Kachi Ghani (Mustard oil), Naturrale (sunflower oil), K.S Brand (Gingelly oil), Parachute (coconut oil), Durga (pure ghee), Vandana (pure ghee) and Himaneesdevee (ghee) purchased from local market Kurnool, Andhrapradesh, India. The determination was done by measuring the absorbance on UV spectrophotometer using Liberman – Burchard reaction. The sterol contents of include Ragava Palm Swathi, Kachi Ghani, Naturrale, K.S Brand, Parachute, determined as 18 mg/gm, 48 mg/g, 56 mg/gm, 84 mg/gm and 13 mg/gm respectively, while for Durga, Vandana and Himaneesdevee found as 30 mg/gm, 29 mg/gm, 23 mg/gm. The more amounts of sterols was found to be in gingelly oil and coconut oil have less in comparison....
A simple, sensitive, precise and specific reverse phase high performance liquid chromatographic method was developed and validated for the simultaneous determination of aspirin and prasugrel hydrochloride in pharmaceutical dosage form. It was found that the excipient in the tablet dosage form does not interfere in the quantification of active drug by proposed method. The HPLC separation was carried out by reverse phase chromatography on Qualisil Gold C8 (5 μm; 250 x 4.6 mm) with a mobile phase composed of 5 mM Phosphate buffer (pH-3.0):Methanol (20:80 v/v) in isocratic mode at a flow rate of 1 ml/min. The detection was monitored at 238 nm. The calibration curve for aspirin and prasugrel HCl was linear from 2 to 18 and 15 to 135 µg/ml. The interday and intraday precision was found to be within limits. The proposed method has adequate sensitivity, reproducibility and specificity for the determination of aspirin and prasugrel HCl in bulk and its tablet dosage forms. LOD and LOQ for aspirin were found to be 10 ng/ml and 30 ng/ml and prasugrel HCl were found to be 30 ng/ml and 100 ng/ml respectively. Accuracy and reproducibility were found satisfactory....
Worldwide need of alternative medicine has resulted into growth of natural product markets and interest in traditional systems of medicine. A study cannot be considered scientifically valid if the material tested was not authenticated and characterized for reproducible result. In the case of botanicals, there may be misidentification of the collected plant, adulteration with other species, or contamination with extraneous ingredients. The scientific integration of herbal medicine into modern medical practices must take into account the interrelated issues of quality, safety and efficacy. Quality is the paramount issue since it can affect the efficacy and/or safety of the herbal products being used. Standardization in each step of development is the need of the hour to ensure the shelf life and product quality. Most of the European country banned many ayurvedic formulations due to the lack of quality and safety. In present study attempt has been made for preparation and evaluation of vidangarista. Four marketed preparations and one in-house preparation were used for the study. The results obtained may be considered as tools for assistance to the regulatory authorities, scientific organizations and manufacturers for developing standards....
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